Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution
- Autores: Kuevda E.1, Gubareva E.1, Gumenyuk I.1, Sotnichenko A.1, Gilevich I.1, Nakokhov R.1, Rusinova T.1, Yudina T.1, Red’ko A.1, Alekseenko S.1
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Afiliações:
- Kuban State Medical University
- Edição: Volume 162, Nº 5 (2017)
- Páginas: 703-706
- Seção: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/238491
- DOI: https://doi.org/10.1007/s10517-017-3692-3
- ID: 238491
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Resumo
We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.
Sobre autores
E. Kuevda
Kuban State Medical University
Autor responsável pela correspondência
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
E. Gubareva
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
I. Gumenyuk
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
A. Sotnichenko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
I. Gilevich
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
R. Nakokhov
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
T. Rusinova
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
T. Yudina
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
A. Red’ko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
S. Alekseenko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Rússia, Krasnodar
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