Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution

E. V. Kuevda; E. A. Gubareva; I. S. Gumenyuk; A. S. Sotnichenko; I. V. Gilevich; R. Z. Nakokhov; T. V. Rusinova; T. G. Yudina; A. N. Red’ko; S. N. Alekseenko

doi:10.1007/s10517-017-3692-3

Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution

Authors: Kuevda E.V.¹, Gubareva E.A.¹, Gumenyuk I.S.¹, Sotnichenko A.S.¹, Gilevich I.V.¹, Nakokhov R.Z.¹, Rusinova T.V.¹, Yudina T.G.¹, Red’ko A.N.¹, Alekseenko S.N.¹
Affiliations:
1. Kuban State Medical University
Issue: Vol 162, No 5 (2017)
Pages: 703-706
Section: Methods
URL: https://journals.rcsi.science/0007-4888/article/view/238491
DOI: https://doi.org/10.1007/s10517-017-3692-3
ID: 238491

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Abstract

We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.