Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution
- Authors: Kuevda E.V.1, Gubareva E.A.1, Gumenyuk I.S.1, Sotnichenko A.S.1, Gilevich I.V.1, Nakokhov R.Z.1, Rusinova T.V.1, Yudina T.G.1, Red’ko A.N.1, Alekseenko S.N.1
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Affiliations:
- Kuban State Medical University
- Issue: Vol 162, No 5 (2017)
- Pages: 703-706
- Section: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/238491
- DOI: https://doi.org/10.1007/s10517-017-3692-3
- ID: 238491
Cite item
Abstract
We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.
About the authors
E. V. Kuevda
Kuban State Medical University
Author for correspondence.
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
E. A. Gubareva
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
I. S. Gumenyuk
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
A. S. Sotnichenko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
I. V. Gilevich
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
R. Z. Nakokhov
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
T. V. Rusinova
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
T. G. Yudina
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
A. N. Red’ko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar
S. N. Alekseenko
Kuban State Medical University
Email: elenakuevda@yandex.ru
Russian Federation, Krasnodar