Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease
- Авторлар: Novosadova E.1, Manuilova E.1, Nenasheva V.1, Tarantul V.1, Grivennikov I.1, Khaspekov L.2, Illarioshkin S.2, Vetchinova A.2, Simonova V.2
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Мекемелер:
- Institute of Molecular Genetics, Russian Academy of Sciences
- Research Center of Neurology
- Шығарылым: Том 165, № 3 (2018)
- Беттер: 378-381
- Бөлім: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/240322
- DOI: https://doi.org/10.1007/s10517-018-4174-y
- ID: 240322
Дәйексөз келтіру
Аннотация
We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.
Авторлар туралы
E. Novosadova
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Ресей, Moscow
E. Manuilova
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Ресей, Moscow
V. Nenasheva
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Ресей, Moscow
V. Tarantul
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Ресей, Moscow
I. Grivennikov
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Ресей, Moscow
L. Khaspekov
Research Center of Neurology
Email: snillario@gmail.com
Ресей, Moscow
S. Illarioshkin
Research Center of Neurology
Хат алмасуға жауапты Автор.
Email: snillario@gmail.com
Ресей, Moscow
A. Vetchinova
Research Center of Neurology
Email: snillario@gmail.com
Ресей, Moscow
V. Simonova
Research Center of Neurology
Email: snillario@gmail.com
Ресей, Moscow