Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease
- Authors: Novosadova E.V.1, Manuilova E.S.1, Nenasheva V.V.1, Tarantul V.Z.1, Grivennikov I.A.1, Khaspekov L.G.2, Illarioshkin S.N.2, Vetchinova A.S.2, Simonova V.V.2
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Affiliations:
- Institute of Molecular Genetics, Russian Academy of Sciences
- Research Center of Neurology
- Issue: Vol 165, No 3 (2018)
- Pages: 378-381
- Section: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/240322
- DOI: https://doi.org/10.1007/s10517-018-4174-y
- ID: 240322
Cite item
Abstract
We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed. These data confirm the importance of the development of new approaches ensuring genome stability in CRISPR/Cas9-edited cultures.
About the authors
E. V. Novosadova
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Russian Federation, Moscow
E. S. Manuilova
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Russian Federation, Moscow
V. V. Nenasheva
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Russian Federation, Moscow
V. Z. Tarantul
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Russian Federation, Moscow
I. A. Grivennikov
Institute of Molecular Genetics, Russian Academy of Sciences
Email: snillario@gmail.com
Russian Federation, Moscow
L. G. Khaspekov
Research Center of Neurology
Email: snillario@gmail.com
Russian Federation, Moscow
S. N. Illarioshkin
Research Center of Neurology
Author for correspondence.
Email: snillario@gmail.com
Russian Federation, Moscow
A. S. Vetchinova
Research Center of Neurology
Email: snillario@gmail.com
Russian Federation, Moscow
V. V. Simonova
Research Center of Neurology
Email: snillario@gmail.com
Russian Federation, Moscow