Comparative Analysis of the Expression of Glutathione Peroxidase and Glutathione Reductase Genes in Human Sperm after Cryopreservation
- Authors: Makarova N.P.1, Romanov Y.A.1, Dolgushina N.V.1, Parker M.M.1, Krasnyi A.M.1
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Affiliations:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 165, No 1 (2018)
- Pages: 166-170
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240118
- DOI: https://doi.org/10.1007/s10517-018-4121-y
- ID: 240118
Cite item
Abstract
We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients. A positive correlation was revealed between GPX1 expression and sperm cryotolerance.
About the authors
N. P. Makarova
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Russian Federation, Moscow
Yu. A. Romanov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: romanov1553@yandex.ru
Russian Federation, Moscow
N. V. Dolgushina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Russian Federation, Moscow
M. M. Parker
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Russian Federation, Moscow
A. M. Krasnyi
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov1553@yandex.ru
Russian Federation, Moscow