Induction of Alternative Splicing and Inhibition of Activity of Telomerase Catalytic Subunit by Apoptotic Endonuclease EndoG in Human T, B, and NK Cells
- Авторлар: Zhdanov D.1, Gladilina Y.1, Pokrovskaya M.1, Aleksandrova S.1, Grishin D.1, Podobed O.1, Sokolov N.1
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Мекемелер:
- Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
- Шығарылым: Том 164, № 4 (2018)
- Беттер: 478-482
- Бөлім: Biotechnology
- URL: https://journals.rcsi.science/0007-4888/article/view/239698
- DOI: https://doi.org/10.1007/s10517-018-4016-y
- ID: 239698
Дәйексөз келтіру
Аннотация
The effect of apoptotic endonuclease EndoG on alternative splicing of mRNA of human telomerase catalytic subunit hTERT (human telomerase reverse transcriptase) and telomerase activity in normal human lymphocytes were studied. Human CD4+, CD8+, B, and NK cells were transfected with a plasmid pEndoG-GFP containing EndoG gene or control plasmid pGFP. The levels of mRNA of EndoG or hTERT splicing variants were analyzed by real-time PCR. Protein content was assessed by Western blotting. Telomerase activity was measured by the telomere repeats amplification protocol. EndoG overexpression reduced the expression of active full-length hTERT and increased the expression of inactive splice variant. Shifted balance of hTERT splice variants in cells led to a significant decrease in telomerase activity within 72 h after transfection.
Авторлар туралы
D. Zhdanov
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Хат алмасуға жауапты Автор.
Email: zhdanovdd@mail.ru
Ресей, Moscow
Yu. Gladilina
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
M. Pokrovskaya
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
S. Aleksandrova
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
D. Grishin
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
O. Podobed
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
N. Sokolov
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Ресей, Moscow
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