Induction of Alternative Splicing and Inhibition of Activity of Telomerase Catalytic Subunit by Apoptotic Endonuclease EndoG in Human T, B, and NK Cells
- Authors: Zhdanov D.D.1, Gladilina Y.A.1, Pokrovskaya M.V.1, Aleksandrova S.S.1, Grishin D.V.1, Podobed O.V.1, Sokolov N.N.1
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Affiliations:
- Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
- Issue: Vol 164, No 4 (2018)
- Pages: 478-482
- Section: Biotechnology
- URL: https://journals.rcsi.science/0007-4888/article/view/239698
- DOI: https://doi.org/10.1007/s10517-018-4016-y
- ID: 239698
Cite item
Abstract
The effect of apoptotic endonuclease EndoG on alternative splicing of mRNA of human telomerase catalytic subunit hTERT (human telomerase reverse transcriptase) and telomerase activity in normal human lymphocytes were studied. Human CD4+, CD8+, B, and NK cells were transfected with a plasmid pEndoG-GFP containing EndoG gene or control plasmid pGFP. The levels of mRNA of EndoG or hTERT splicing variants were analyzed by real-time PCR. Protein content was assessed by Western blotting. Telomerase activity was measured by the telomere repeats amplification protocol. EndoG overexpression reduced the expression of active full-length hTERT and increased the expression of inactive splice variant. Shifted balance of hTERT splice variants in cells led to a significant decrease in telomerase activity within 72 h after transfection.
About the authors
D. D. Zhdanov
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Author for correspondence.
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
Yu. A. Gladilina
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
M. V. Pokrovskaya
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
S. S. Aleksandrova
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
D. V. Grishin
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
O. V. Podobed
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow
N. N. Sokolov
Laboratory of Medical Biotechnology, V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: zhdanovdd@mail.ru
Russian Federation, Moscow