Effects of Quercetin on Expression of Genes of Carbohydrate and Lipid Metabolism Enzymes in the Liver of Rats Receiving High-Fructose Ration
- Authors: Mzhel’skaya K.V.1, Trusov N.V.1, Guseva G.N.1, Aksenov I.V.1, Kravchenko L.V.1, Tutelyan V.A.1
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Affiliations:
- Federal Research Center of Nutrition and Biotechnology
- Issue: Vol 167, No 2 (2019)
- Pages: 263-266
- Section: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/241634
- DOI: https://doi.org/10.1007/s10517-019-04505-0
- ID: 241634
Cite item
Abstract
We studied the expression of genes encoding enzymes of carbohydrate and lipid metabolism ketohexokinase (Khk), glucokinase (Gck), pyruvate kinase (Pklr), acetyl-Co-carboxylase (Acaca), fatty acid synthase (Fasn), stearoyl-CoA desaturase (Scd), and their transcription regulators ChREBP (Mlxipl), SREBP-1c (Srebf1), and PPARα (Ppara) in rat liver. Control group rats received a semisynthetic ration over 20 weeks. Experimental group 1 received a semisynthetic ration and 20% fructose solution instead of drinking water. Experimental group 2 rats received a semisynthetic ration with quercetin (0.1% fodder weight) and 20% fructose solution. Consumption of 20% fructose solution (experimental group 1) led to an increase in Scd expression in comparison with the control and did not affect the expression of other genes. Addition of quercetin to the ration (experimental group 2) led to a decrease in the expression of Khk, Gck, Fasn, Scd, Mlxipl, and Ppara genes in comparison with experimental group 1. The results suggest that quercetin reduced the expression of genes of carbohydrate and lipid metabolism enzymes in the liver of rats receiving high-fructose ration.
About the authors
K. V. Mzhel’skaya
Federal Research Center of Nutrition and Biotechnology
Author for correspondence.
Email: kristik13@yandex.ru
Russian Federation, Moscow
N. V. Trusov
Federal Research Center of Nutrition and Biotechnology
Email: kristik13@yandex.ru
Russian Federation, Moscow
G. N. Guseva
Federal Research Center of Nutrition and Biotechnology
Email: kristik13@yandex.ru
Russian Federation, Moscow
I. V. Aksenov
Federal Research Center of Nutrition and Biotechnology
Email: kristik13@yandex.ru
Russian Federation, Moscow
L. V. Kravchenko
Federal Research Center of Nutrition and Biotechnology
Email: kristik13@yandex.ru
Russian Federation, Moscow
V. A. Tutelyan
Federal Research Center of Nutrition and Biotechnology
Email: kristik13@yandex.ru
Russian Federation, Moscow