Antisense Oligonucleotides Immobilized on Silicon-Organic Nanoparticles as a Tool for Prolonged Correction of Hypertensive States
- Authors: Levina A.S.1,2, Repkova M.N.1,2, Klimov L.O.1,3, Ryazanova M.A.1,3, Markel A.L.1,3, Zarytova V.F.1,2
-
Affiliations:
- Novosibirsk State University
- Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
- Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
- Issue: Vol 167, No 1 (2019)
- Pages: 116-119
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241497
- DOI: https://doi.org/10.1007/s10517-019-04473-5
- ID: 241497
Cite item
Abstract
We propose an original method for controlling BP by administration of Si~ODN nanocomposites containing antisense oligonucleotides fixed on silicon-organic nanoparticles. ODN in nanocomposites are targeted to mRNA of the genes encoding angiotensin-converting enzyme (ACE1) and type 1 angiotensin-II receptor (AT1A). The experiments were performed on hypertensive ISIAH rats, a genetic model of hypertension. Single inhalation or intraperitoneal administration of the nanocomposites targeted to ACE1 mRNA or ATA1 mRNA, respectively, led to a pronounced decrease (by ~30 mm Hg) in systolic BP in ISIAH rats over a week. The use of scrambled ODN in the nanocomposites had no effect. A decrease in the expression of ACE1 and AT1A genes under the effect of the corresponding antisense ODN was demonstrated, which attested to directed effect of the test preparations.
About the authors
A. S. Levina
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Author for correspondence.
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk
M. N. Repkova
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk
L. O. Klimov
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk
M. A. Ryazanova
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk
A. L. Markel
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk
V. F. Zarytova
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Russian Federation, Novosibirsk; Novosibirsk