Antisense Oligonucleotides Immobilized on Silicon-Organic Nanoparticles as a Tool for Prolonged Correction of Hypertensive States
- Autores: Levina A.1,2, Repkova M.1,2, Klimov L.1,3, Ryazanova M.1,3, Markel A.1,3, Zarytova V.1,2
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Afiliações:
- Novosibirsk State University
- Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
- Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
- Edição: Volume 167, Nº 1 (2019)
- Páginas: 116-119
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241497
- DOI: https://doi.org/10.1007/s10517-019-04473-5
- ID: 241497
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Resumo
We propose an original method for controlling BP by administration of Si~ODN nanocomposites containing antisense oligonucleotides fixed on silicon-organic nanoparticles. ODN in nanocomposites are targeted to mRNA of the genes encoding angiotensin-converting enzyme (ACE1) and type 1 angiotensin-II receptor (AT1A). The experiments were performed on hypertensive ISIAH rats, a genetic model of hypertension. Single inhalation or intraperitoneal administration of the nanocomposites targeted to ACE1 mRNA or ATA1 mRNA, respectively, led to a pronounced decrease (by ~30 mm Hg) in systolic BP in ISIAH rats over a week. The use of scrambled ODN in the nanocomposites had no effect. A decrease in the expression of ACE1 and AT1A genes under the effect of the corresponding antisense ODN was demonstrated, which attested to directed effect of the test preparations.
Sobre autores
A. Levina
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Autor responsável pela correspondência
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
M. Repkova
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
L. Klimov
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
M. Ryazanova
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
A. Markel
Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
V. Zarytova
Novosibirsk State University; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: levina@niboch.nsc.ru
Rússia, Novosibirsk; Novosibirsk
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