Abnormal Membrane Localization of α2 Isoform of Na,K-ATPase in m. soleus of Dysferlin-Deficient Mice
- Authors: Kravtsova V.V.1, Bouzinova E.V.2, Machkov V.V.2, Timonina N.A.1, Zakyrjanova G.F.3,4, Zefirov A.L.4, Krivoi I.I.1
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Affiliations:
- Department of General Physiology, St. Petersburg State University
- Department of Biomedicine, Health, Aarhus University
- Kazan Institute of Biochemistry and Biophysics, Federal Research Center Kazan Scientific Center of the Russian Academy of Science
- Department of Normal Physiology, Kazan State Medical University
- Issue: Vol 166, No 5 (2019)
- Pages: 593-597
- Section: Physiology
- URL: https://journals.rcsi.science/0007-4888/article/view/241217
- DOI: https://doi.org/10.1007/s10517-019-04398-z
- ID: 241217
Cite item
Abstract
Dysferlin protein plays a key role in the multimolecular complex responsible for the maintenance of sarcolemma integrity and skeletal muscle cell functioning. We studied the membrane distribution of nicotinic acetylcholine receptors and α2 isoform of Na,K-ATPase in motor endplates of m. soleus in dysferlin-deficient Bla/J mice (a dysferlinopathy model). Endplates of Bla/J mice were characterized by increased area (without changes in fragmentation degree) and reduced density of the membrane distribution of nicotinic acetylcholine receptors in comparison with the corresponding parameters in control С57Bl/6 mice. The density of the membrane distribution of α2 isoform of Na,K-ATPase was also reduced, but the level of the corresponding mRNA remained unchanged. It can be hypothesized that abnormal membrane localization of α2 isoform of Na,K-ATPase results from adaptive skeletal muscle remodeling under conditions of chronic motor dysfunction.
About the authors
V. V. Kravtsova
Department of General Physiology, St. Petersburg State University
Email: iikrivoi@gmail.com
Russian Federation, St. Petersburg
E. V. Bouzinova
Department of Biomedicine, Health, Aarhus University
Email: iikrivoi@gmail.com
Denmark, Aarhus
V. V. Machkov
Department of Biomedicine, Health, Aarhus University
Email: iikrivoi@gmail.com
Denmark, Aarhus
N. A. Timonina
Department of General Physiology, St. Petersburg State University
Email: iikrivoi@gmail.com
Russian Federation, St. Petersburg
G. F. Zakyrjanova
Kazan Institute of Biochemistry and Biophysics, Federal Research Center Kazan Scientific Center of the Russian Academy of Science; Department of Normal Physiology, Kazan State Medical University
Email: iikrivoi@gmail.com
Russian Federation, Kazan; Kazan, Tatarstan Republic
A. L. Zefirov
Department of Normal Physiology, Kazan State Medical University
Email: iikrivoi@gmail.com
Russian Federation, Kazan, Tatarstan Republic
I. I. Krivoi
Department of General Physiology, St. Petersburg State University
Author for correspondence.
Email: iikrivoi@gmail.com
Russian Federation, St. Petersburg