Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia
- Authors: Dzhalilova D.S.1, Diatroptov M.E.1, Tsvetkov I.S.1, Makarova O.V.1, Kuznetsov S.L.2
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Affiliations:
- Research Institute of Human Morphology
- I. M. Sechenov First Moscow State Medical University
- Issue: Vol 165, No 6 (2018)
- Pages: 781-785
- Section: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/240748
- DOI: https://doi.org/10.1007/s10517-018-4264-x
- ID: 240748
Cite item
Abstract
We studied the expression of Hif-1α, Nf-κb, and Vegf genes in the liver and serum levels of HIF-1α, erythropoietin, VEGF, TGF-β, 8-isoprostane, and corticosterone in Wistar rats with different resistance to hypoxia in 5 and 90 min after acute exposure to hypobaric hypoxia. In 5 min after hypoxic exposure, Hif-1α expression in the liver and serum levels of erythropoietin, VEGF, and TGF-β in high-resistant rats were higher than in low-resistant animals. In highresistant rats, the increment in expression of Nf-κb gene responsible for the control over the inflammatory processes was more pronounced than in low-resistant animals. In 90 min after hypoxic exposure, the serum levels of HIF-1α, erythropoietin, VEGF, and TGF-β returned to normal in high-resistant rats, while in low-resistant animals, an increase in 8-isoprostane and TGF-β concentrations was observed. The rats with different resistance to hypoxia were characterized by different changes in biomolecular parameters determining predilection to inflammatory diseases.
About the authors
D. S. Dzhalilova
Research Institute of Human Morphology
Author for correspondence.
Email: juliajal93@mail.ru
Russian Federation, Moscow
M. E. Diatroptov
Research Institute of Human Morphology
Email: juliajal93@mail.ru
Russian Federation, Moscow
I. S. Tsvetkov
Research Institute of Human Morphology
Email: juliajal93@mail.ru
Russian Federation, Moscow
O. V. Makarova
Research Institute of Human Morphology
Email: juliajal93@mail.ru
Russian Federation, Moscow
S. L. Kuznetsov
I. M. Sechenov First Moscow State Medical University
Email: juliajal93@mail.ru
Russian Federation, Moscow