Effect of Native and Modified Apolipoprotein A-I on DNA Synthesis in Cultures of Different Cells
- Authors: Usynin I.F.1, Dudarev A.N.1, Miroshnichenko S.M.1, Tkachenko T.A.1, Gorodetskaya A.Y.1
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Affiliations:
- Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
- Issue: Vol 164, No 2 (2017)
- Pages: 247-251
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239535
- DOI: https://doi.org/10.1007/s10517-017-3967-8
- ID: 239535
Cite item
Abstract
Culturing of bone marrow cells in serum-free RPMI-1640 medium for 24 h was accompanied by a decrease in the rate of [3H]-thymidine incorporation into DNA. Addition of native apolipoprotein A-I (apoA-I) or plasma LDL and HDL to the culture medium increased this parameter. In contrast to native apoA-I, its modified form decelerated DNA synthesis in bone marrow cells. A similar inhibitory effect of modified protein was observed in cultures of human embryonic kidney cells (HEK293) and in rapidly proliferating mouse macrophage cell line ANA-1. The only exclusion was human myeloid cell line U937: neither native nor modified apoA-I affected DNA synthesis in these cells. Thus, the regulatory effects of apoA-I are tissue-specific; this protein can produce either stimulatory or inhibitory effect on DNA biosynthesis in cells depending on its conformation.
About the authors
I. F. Usynin
Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
Author for correspondence.
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
A. N. Dudarev
Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
S. M. Miroshnichenko
Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
T. A. Tkachenko
Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
A. Yu. Gorodetskaya
Laboratory of Mechanisms of Intercellular Interactions, Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk