Possibility of Aggravation of Tissue Sclerosis after Injection of Multipotent Mesenchymal Stromal Cells Near the Forming Cicatrix in the Experiment
- Authors: Maiborodin I.V.1, Morozov V.V.1, Anikeev A.A.1, Figurenko N.F.1, Maslov R.V.1, Matveeva V.A.1, Chastikina G.A.1, Maiborodina V.I.2
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Affiliations:
- Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
- Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
- Issue: Vol 163, No 4 (2017)
- Pages: 554-560
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239085
- DOI: https://doi.org/10.1007/s10517-017-3848-1
- ID: 239085
Cite item
Abstract
The peculiarities of tissue sclerosis after injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained with Vybrant CM-Dil cell membrane dye were studied by light microscopy with luminescence. The surgical intervention consisting in ligation of the great vein was followed by tissue sclerotic transformation caused by direct damage and chronic inflammation caused by the presence of slowly resorbed ligature. Injection of stromal cells after this intervention led to formation of more extensive scar. This can attest to the possibility of stromal cells differentiation into connective tissue cells, fibroblasts, and stimulation of proliferation and collagen synthesis by host fibroblasts. A decrease in the volume of dense fibrous connective tissue due to scar reorganization at latter terms cannot not excluded.
About the authors
I. V. Maiborodin
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Author for correspondence.
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. V. Morozov
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
A. A. Anikeev
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
N. F. Figurenko
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
R. V. Maslov
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. A. Matveeva
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
G. A. Chastikina
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences
Email: imai@mail.ru
Russian Federation, Novosibirsk
V. I. Maiborodina
Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology
Email: imai@mail.ru
Russian Federation, Novosibirsk