Involvement of Protein Kinase C-δ in the Realization of Cardioprotective Effect of Ischemic Postconditioning
- Authors: Lishmanov Y.B.1, Gorbunov A.S.1, Maslov L.N.1
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Affiliations:
- Research Institute of Cardiology
- Issue: Vol 161, No 3 (2016)
- Pages: 344-346
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237405
- DOI: https://doi.org/10.1007/s10517-016-3410-6
- ID: 237405
Cite item
Abstract
Experiments on isolated perfused rat heart modeled 45-min global ischemia followed by 30-min reperfusion. Ischemic postconditioning was modeled by 3 cycles of reperfusion (30 sec) and ischemia (30 sec). Cardiomyocyte necrosis was assessed by the level of creatine phosphokinase in the perfusate. Postconditioning reduced the release of creatine phosphokinase from the heart by 30%. The cardioprotective effect of ischemic postconditioning was eliminated after inhibition of protein kinase C with cheleritrin or after blockade of δ-isoform of protein kinase C with rottlerin. These findings attest to participation of protein kinase C-δ in the realization of the cardioprotective effect of postconditioning.
About the authors
Yu. B. Lishmanov
Research Institute of Cardiology
Email: maslov@cardio-tomsk.ru
Russian Federation, Tomsk
A. S. Gorbunov
Research Institute of Cardiology
Email: maslov@cardio-tomsk.ru
Russian Federation, Tomsk
L. N. Maslov
Research Institute of Cardiology
Author for correspondence.
Email: maslov@cardio-tomsk.ru
Russian Federation, Tomsk