Effect of Peptide AEDG on Telomere Length and Mitotic Index of PHA-Stimulated Human Blood Lymphocytes
- Authors: Khavinson V.K.1, Pendina A.A.2, Efimova O.A.2, Tikhonov A.V.2, Koltsova A.S.2, Krapivin M.I.2, Petrovskaia-Kaminskaia A.V.2, Petrova L.I.2, Lin’kova N.S.1,3, Baranov V.S.2
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Affiliations:
- Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology
- Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- Department of Therapy, Geriatry, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research Clinical Center, Federal Medical-Biological Agency of Russia
- Issue: Vol 168, No 1 (2019)
- Pages: 141-144
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/242286
- DOI: https://doi.org/10.1007/s10517-019-04664-0
- ID: 242286
Cite item
Abstract
We studied the effect of peptide AEDG on telomere length and mitotic index of PHA-stimulated blood lymphocytes from young (18-22 years, N=5) and middle-aged (49-54 years, N=6) men. In the younger age group, no significant changes in the mitotic index were detected, while in the middle-aged group, a decrease in this parameter was found in one case. The relative length of telomeric regions of metaphase chromosomes was evaluated by in situ fluorescence hybridization with DNA probes specific to telomeres. After incubation with peptide AEDG, significant changes in the relative telomere length were found in 7 of 11 individuals (3 cases in the younger age group and 4 cases in the middle age group). Significant increase in telomere length after exposure to peptide AEDG was revealed in 5 cases, including two individuals of the younger age group (by 41 and 55%) and three individuals of the middle age group (by 156, 18, and 76%). In one individual of the younger age group and in one of the middle-age group, a significant decrease in telomere length (by 37 and 15%, respectively) was found. A tendency to normalization of telomere lengths was noted: this parameter increased in individuals with initially lower telomere length relative to the group mean value and decreased in individuals with initially longer telomeres compared to the mean length in the group.
About the authors
V. Kh. Khavinson
Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology
Email: miayy@yandex.ru
Russian Federation, Moscow
A. A. Pendina
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
O. A. Efimova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
A. V. Tikhonov
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
A. S. Koltsova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
M. I. Krapivin
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
A. V. Petrovskaia-Kaminskaia
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
L. I. Petrova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg
N. S. Lin’kova
Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology; Department of Therapy, Geriatry, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research Clinical Center, Federal Medical-Biological Agency of Russia
Author for correspondence.
Email: miayy@yandex.ru
Russian Federation, Moscow; Moscow
V. S. Baranov
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Russian Federation, St. Petersburg