Effect of Peptide AEDG on Telomere Length and Mitotic Index of PHA-Stimulated Human Blood Lymphocytes
- Autores: Khavinson V.1, Pendina A.2, Efimova O.2, Tikhonov A.2, Koltsova A.2, Krapivin M.2, Petrovskaia-Kaminskaia A.2, Petrova L.2, Lin’kova N.1,3, Baranov V.2
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Afiliações:
- Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology
- Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- Department of Therapy, Geriatry, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research Clinical Center, Federal Medical-Biological Agency of Russia
- Edição: Volume 168, Nº 1 (2019)
- Páginas: 141-144
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/242286
- DOI: https://doi.org/10.1007/s10517-019-04664-0
- ID: 242286
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Resumo
We studied the effect of peptide AEDG on telomere length and mitotic index of PHA-stimulated blood lymphocytes from young (18-22 years, N=5) and middle-aged (49-54 years, N=6) men. In the younger age group, no significant changes in the mitotic index were detected, while in the middle-aged group, a decrease in this parameter was found in one case. The relative length of telomeric regions of metaphase chromosomes was evaluated by in situ fluorescence hybridization with DNA probes specific to telomeres. After incubation with peptide AEDG, significant changes in the relative telomere length were found in 7 of 11 individuals (3 cases in the younger age group and 4 cases in the middle age group). Significant increase in telomere length after exposure to peptide AEDG was revealed in 5 cases, including two individuals of the younger age group (by 41 and 55%) and three individuals of the middle age group (by 156, 18, and 76%). In one individual of the younger age group and in one of the middle-age group, a significant decrease in telomere length (by 37 and 15%, respectively) was found. A tendency to normalization of telomere lengths was noted: this parameter increased in individuals with initially lower telomere length relative to the group mean value and decreased in individuals with initially longer telomeres compared to the mean length in the group.
Sobre autores
V. Khavinson
Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology
Email: miayy@yandex.ru
Rússia, Moscow
A. Pendina
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
O. Efimova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
A. Tikhonov
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
A. Koltsova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
M. Krapivin
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
A. Petrovskaia-Kaminskaia
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
L. Petrova
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg
N. Lin’kova
Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology; Department of Therapy, Geriatry, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research Clinical Center, Federal Medical-Biological Agency of Russia
Autor responsável pela correspondência
Email: miayy@yandex.ru
Rússia, Moscow; Moscow
V. Baranov
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: miayy@yandex.ru
Rússia, St. Petersburg