Induction of Osteogenesis in Rat Bone Tissue Using Cryogenically Structured Porous 3D Materials Containing a Bioregulator

Abstract

We studied osteogenesis induction after implantation of porous cryogenically structured 3D scaffolds based on chitosan, alginate, and serum albumin and containing a bioregulator from bovine serum into in vivo modeled experimental bone defect in rats. It was shown that such 3D-matrices are effective as osteoconductors due to their effect on osteoblast precursors. All types of bioregulator-loaded 3D materials promote active bone repair, which manifested in restoration of the dense bone tissue, formation of the bone marrow, and the recovery of osteons on day 14 after surgery. In animals receiving implantation of control 3D scaffolds without the bioregulator, the formation of dense fibrous tissue and spongy bone was observed by this term.