Transport of Human Lactoferrin into Mouse Brain: Administration Routes and Distribution
- Authors: Kopaeva Y.1, Cherepov A.B.1, Zarayskaya I.Y.1, Nesterenko M.V.2
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Affiliations:
- National Research Center Kurchatov Institute
- Lactobio Company
- Issue: Vol 167, No 4 (2019)
- Pages: 561-567
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241894
- DOI: https://doi.org/10.1007/s10517-019-04572-3
- ID: 241894
Cite item
Abstract
We studied different ways of transport of human lactoferrin to the brain of C57Bl/6 mice after its administration via different routes, analyzed its distribution in the brain, and determined the phenotype of lactoferrin-containing cells. Colocalization of lactoferrin and markers of various cell types was estimated by fluorescent immunohistochemical analysis. Lactoferrin was detected in mouse brain sections after its intranasal, sublingual, and intraperitoneal administration, but not after conjunctival administration. After intranasal administration, lactoferrin rapidly penetrated into the brain and accumulated in the cytoplasm of vascular endothelial cells in the neocortex, striatum, hippocampus, and thalamus. After application of protein solution onto fixed floating sections, highly specific binding of lactoferrin was found in the nuclei of neurons, astrocytes, and microglia cells, but not in the nuclei of endothelial cells of mouse brain.
About the authors
Yu. Kopaeva
National Research Center Kurchatov Institute
Author for correspondence.
Email: kopaeva_my@nrcki.ru
Russian Federation, Moscow
A. B. Cherepov
National Research Center Kurchatov Institute
Email: kopaeva_my@nrcki.ru
Russian Federation, Moscow
I. Yu. Zarayskaya
National Research Center Kurchatov Institute
Email: kopaeva_my@nrcki.ru
Russian Federation, Moscow
M. V. Nesterenko
Lactobio Company
Email: kopaeva_my@nrcki.ru
Russian Federation, Moscow
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