Transport of Human Lactoferrin into Mouse Brain: Administration Routes and Distribution
- 作者: Kopaeva Y.1, Cherepov A.1, Zarayskaya I.1, Nesterenko M.2
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隶属关系:
- National Research Center Kurchatov Institute
- Lactobio Company
- 期: 卷 167, 编号 4 (2019)
- 页面: 561-567
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241894
- DOI: https://doi.org/10.1007/s10517-019-04572-3
- ID: 241894
如何引用文章
详细
We studied different ways of transport of human lactoferrin to the brain of C57Bl/6 mice after its administration via different routes, analyzed its distribution in the brain, and determined the phenotype of lactoferrin-containing cells. Colocalization of lactoferrin and markers of various cell types was estimated by fluorescent immunohistochemical analysis. Lactoferrin was detected in mouse brain sections after its intranasal, sublingual, and intraperitoneal administration, but not after conjunctival administration. After intranasal administration, lactoferrin rapidly penetrated into the brain and accumulated in the cytoplasm of vascular endothelial cells in the neocortex, striatum, hippocampus, and thalamus. After application of protein solution onto fixed floating sections, highly specific binding of lactoferrin was found in the nuclei of neurons, astrocytes, and microglia cells, but not in the nuclei of endothelial cells of mouse brain.
作者简介
Yu. Kopaeva
National Research Center Kurchatov Institute
编辑信件的主要联系方式.
Email: kopaeva_my@nrcki.ru
俄罗斯联邦, Moscow
A. Cherepov
National Research Center Kurchatov Institute
Email: kopaeva_my@nrcki.ru
俄罗斯联邦, Moscow
I. Zarayskaya
National Research Center Kurchatov Institute
Email: kopaeva_my@nrcki.ru
俄罗斯联邦, Moscow
M. Nesterenko
Lactobio Company
Email: kopaeva_my@nrcki.ru
俄罗斯联邦, Moscow
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