Email this article Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages
- Authors: Pirutin S.K.1,2, Efremova M.V.1,3, Yusipovich A.I.1, Turovetskii V.B.1, Maksimov G.V.1, Druzhko A.B.2, Mazhuga A.G.1,3,4
-
Affiliations:
- M. V. Lomonosov Moscow State University
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- National University of Science and Technology MISIS
- D. I. Mendeleev University of Chemical Technology of Russia
- Issue: Vol 166, No 3 (2019)
- Pages: 386-389
- Section: Biotechnologies
- URL: https://journals.rcsi.science/0007-4888/article/view/241026
- DOI: https://doi.org/10.1007/s10517-019-04356-9
- ID: 241026
Cite item
Open Access
Access granted
Subscription Access
Abstract
We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.
About the authors
S. K. Pirutin
M. V. Lomonosov Moscow State University; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Author for correspondence.
Email: pirutin@yandex.ru
Russian Federation, Moscow; Pushchino, Moscow Region
M. V. Efremova
M. V. Lomonosov Moscow State University; National University of Science and Technology MISIS
Email: pirutin@yandex.ru
Russian Federation, Moscow; Moscow
A. I. Yusipovich
M. V. Lomonosov Moscow State University
Email: pirutin@yandex.ru
Russian Federation, Moscow
V. B. Turovetskii
M. V. Lomonosov Moscow State University
Email: pirutin@yandex.ru
Russian Federation, Moscow
G. V. Maksimov
M. V. Lomonosov Moscow State University
Email: pirutin@yandex.ru
Russian Federation, Moscow
A. B. Druzhko
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: pirutin@yandex.ru
Russian Federation, Pushchino, Moscow Region
A. G. Mazhuga
M. V. Lomonosov Moscow State University; National University of Science and Technology MISIS; D. I. Mendeleev University of Chemical Technology of Russia
Email: pirutin@yandex.ru
Russian Federation, Moscow; Moscow; Moscow
