Effect of α2-Adrenoceptor Stimulation on Functional Parameters of Langendorff-Isolated Rat Heart

Abstract

We studied the effect of α₂-adrenoreceptor agonist clonidine hydrochloride in concentrations of 10^—9-10^—6 M on inotropy, chronotropy, and coronary flow in Langendorff-isolated heart of adult rats. It was found that α₂-adrenoreceptor agonist changed all studied parameters. Left ventricular myocardium contraction force decreased after application of all tested concentrations, the maximum effect was observed at a concentration of 10^—6 M. Stimulation of α₂-adrenergic receptors in concentrations of 10^—8, 10^—7, and 10^—6 M produced a two-phase effect (initial increase and a subsequent decrease) on the coronary flow. Clonidine hydrochloride in the maximum concentration (10^—6 M) caused a decrease in HR in one group and an increase in the other.