Optimization of Single-Step Assay for Circulating Nitrite and Nitrate Ions (NOx) as Risk Factors of Cardiovascular Mortality
- Authors: Gumanova N.G.1, Klimushina M.V.1, Metel’skaya V.A.1
-
Affiliations:
- National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
- Issue: Vol 165, No 2 (2018)
- Pages: 284-287
- Section: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/240232
- DOI: https://doi.org/10.1007/s10517-018-4149-z
- ID: 240232
Cite item
Abstract
We propose an optimized single-step assay of total nitrite and nitrate concentrations based on serum deproteinization by ultrafiltration. After deproteinization, the residual protein concentration was 0.0800±0.0007 mg/ml, which secured necessary transparency of the specimens in contrast to methanol deproteinization. The optimized assay for serum nitrite and nitrate ions is characterized by high reproducibility and accuracy (variation coefficient <7%) and can be recommended for routine use in clinical practice.
About the authors
N. G. Gumanova
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Email: mklimushina@gmail.com
Russian Federation, Moscow
M. V. Klimushina
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mklimushina@gmail.com
Russian Federation, Moscow
V. A. Metel’skaya
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Email: mklimushina@gmail.com
Russian Federation, Moscow