Optimization of Single-Step Assay for Circulating Nitrite and Nitrate Ions (NOx) as Risk Factors of Cardiovascular Mortality
- Autores: Gumanova N.1, Klimushina M.1, Metel’skaya V.1
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Afiliações:
- National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
- Edição: Volume 165, Nº 2 (2018)
- Páginas: 284-287
- Seção: Methods
- URL: https://journals.rcsi.science/0007-4888/article/view/240232
- DOI: https://doi.org/10.1007/s10517-018-4149-z
- ID: 240232
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Resumo
We propose an optimized single-step assay of total nitrite and nitrate concentrations based on serum deproteinization by ultrafiltration. After deproteinization, the residual protein concentration was 0.0800±0.0007 mg/ml, which secured necessary transparency of the specimens in contrast to methanol deproteinization. The optimized assay for serum nitrite and nitrate ions is characterized by high reproducibility and accuracy (variation coefficient <7%) and can be recommended for routine use in clinical practice.
Sobre autores
N. Gumanova
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Email: mklimushina@gmail.com
Rússia, Moscow
M. Klimushina
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: mklimushina@gmail.com
Rússia, Moscow
V. Metel’skaya
National Research Center for Preventive Medicine, Ministry of Health of the Russian Federation
Email: mklimushina@gmail.com
Rússia, Moscow