Binding of Mucin by E. coli from Human Gut
- Authors: Vakhrusheva T.V.1, Baikova Y.P.1, Balabushevich N.G.1, Gusev S.A.1, Lomakina G.Y.2, Sholina E.A.2, Moshkovskaya M.A.1, Shcherbakov P.L.1, Pobeguts O.V.1, Mikhal’chik E.V.1
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Affiliations:
- Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- Issue: Vol 165, No 2 (2018)
- Pages: 235-238
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240190
- DOI: https://doi.org/10.1007/s10517-018-4137-3
- ID: 240190
Cite item
Abstract
Cells of E. coli isolates from the gut of healthy volunteers (N=5) and patients with Crohn’s disease (N=5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorption of mucin solution at 214 nm after incubation with bacteria. Detailed analysis of mucin binding by one of isolates showed that during incubation of 0.09 mg/ml bacteria in 0.15 M NaCl containing 0.1 mg/ml mucin at 25oC, maximum binding was reached in 30 min, while in the presence of 14 mM α-methyl mannoside, mucin binding decreased by 46% (p<0.05). Confocal microscopy revealed intensive binding of FITC-labeled mucin to the surface of a small number of bacterial cells. Mucin binding did not significantly affect zeta potential of bacteria and their energetic status assessed by ATP content; at the same time, ATP content in the extracellular environment slightly increased.
About the authors
T. V. Vakhrusheva
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
Yu. P. Baikova
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
N. G. Balabushevich
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
S. A. Gusev
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
G. Yu. Lomakina
Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: lemik2007@yandex.ru
Russian Federation, Moscow
E. A. Sholina
Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: lemik2007@yandex.ru
Russian Federation, Moscow
M. A. Moshkovskaya
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
P. L. Shcherbakov
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
O. V. Pobeguts
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
Russian Federation, Moscow
E. V. Mikhal’chik
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Author for correspondence.
Email: lemik2007@yandex.ru
Russian Federation, Moscow