Binding of Mucin by E. coli from Human Gut
- 作者: Vakhrusheva T.1, Baikova Y.1, Balabushevich N.1, Gusev S.1, Lomakina G.2, Sholina E.2, Moshkovskaya M.1, Shcherbakov P.1, Pobeguts O.1, Mikhal’chik E.1
-
隶属关系:
- Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- 期: 卷 165, 编号 2 (2018)
- 页面: 235-238
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240190
- DOI: https://doi.org/10.1007/s10517-018-4137-3
- ID: 240190
如何引用文章
详细
Cells of E. coli isolates from the gut of healthy volunteers (N=5) and patients with Crohn’s disease (N=5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding was evaluated by the decrease in optical absorption of mucin solution at 214 nm after incubation with bacteria. Detailed analysis of mucin binding by one of isolates showed that during incubation of 0.09 mg/ml bacteria in 0.15 M NaCl containing 0.1 mg/ml mucin at 25oC, maximum binding was reached in 30 min, while in the presence of 14 mM α-methyl mannoside, mucin binding decreased by 46% (p<0.05). Confocal microscopy revealed intensive binding of FITC-labeled mucin to the surface of a small number of bacterial cells. Mucin binding did not significantly affect zeta potential of bacteria and their energetic status assessed by ATP content; at the same time, ATP content in the extracellular environment slightly increased.
作者简介
T. Vakhrusheva
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
Yu. Baikova
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
N. Balabushevich
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
S. Gusev
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
G. Lomakina
Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
E. Sholina
Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
M. Moshkovskaya
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
P. Shcherbakov
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
O. Pobeguts
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow
E. Mikhal’chik
Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency
编辑信件的主要联系方式.
Email: lemik2007@yandex.ru
俄罗斯联邦, Moscow