Real-Time Recording of Platelet Adhesion to Fibrinogen-Coated Surface under Flow Conditions
- Authors: Avtaeva Y.N.1,2, Mel’nikov I.S.2, Gabbasov Z.A.2
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Affiliations:
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- Issue: Vol 165, No 1 (2018)
- Pages: 157-160
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240097
- DOI: https://doi.org/10.1007/s10517-018-4119-5
- ID: 240097
Cite item
Abstract
We present a testing system allowing real-time recording of the kinetics of platelet adhesion to fibrinogen-coated surface under flow conditions. The system consists of an optical flow chamber, semiconductor laser, two photodetectors, analog-to-digital converter, computer, and peristaltic pump. Platelet adhesion to fibrinogen-coated surface is recorded with two photodetectors and analyzed by the intensity of total internal reflection and scattered laser radiation at the boundary of the blood sample and fibrinogen-coated optical surface. Kinetics of platelet adhesion was studied as a function of shear rate and platelet concentration. The specificity of platelet adhesion with proteins on the surface of the flow chamber was verified by blocking IIb/IIIa glycoprotein complex on platelets with Fab2 fragments of monoclonal antibodies.
About the authors
Yu. N. Avtaeva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: julia_94fs@mail.ru
Russian Federation, Moscow; Moscow
I. S. Mel’nikov
Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: julia_94fs@mail.ru
Russian Federation, Moscow
Z. A. Gabbasov
Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: julia_94fs@mail.ru
Russian Federation, Moscow