Real-Time Recording of Platelet Adhesion to Fibrinogen-Coated Surface under Flow Conditions
- Авторлар: Avtaeva Y.1,2, Mel’nikov I.2, Gabbasov Z.2
-
Мекемелер:
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- Шығарылым: Том 165, № 1 (2018)
- Беттер: 157-160
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240097
- DOI: https://doi.org/10.1007/s10517-018-4119-5
- ID: 240097
Дәйексөз келтіру
Аннотация
We present a testing system allowing real-time recording of the kinetics of platelet adhesion to fibrinogen-coated surface under flow conditions. The system consists of an optical flow chamber, semiconductor laser, two photodetectors, analog-to-digital converter, computer, and peristaltic pump. Platelet adhesion to fibrinogen-coated surface is recorded with two photodetectors and analyzed by the intensity of total internal reflection and scattered laser radiation at the boundary of the blood sample and fibrinogen-coated optical surface. Kinetics of platelet adhesion was studied as a function of shear rate and platelet concentration. The specificity of platelet adhesion with proteins on the surface of the flow chamber was verified by blocking IIb/IIIa glycoprotein complex on platelets with Fab2 fragments of monoclonal antibodies.
Авторлар туралы
Yu. Avtaeva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Хат алмасуға жауапты Автор.
Email: julia_94fs@mail.ru
Ресей, Moscow; Moscow
I. Mel’nikov
Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: julia_94fs@mail.ru
Ресей, Moscow
Z. Gabbasov
Laboratory of Stem Cells, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: julia_94fs@mail.ru
Ресей, Moscow