Email this article Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries
- Authors: Voronova А.D.1,2, Stepanova O.V.2, Valikhov M.P.2, Chadin A.V.2, Dvornikov А.S.2, Reshetov I.V.3, Chekhonin V.P.1,2
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Affiliations:
- Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
- University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 4 (2018)
- Pages: 523-527
- Section: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/239730
- DOI: https://doi.org/10.1007/s10517-018-4025-x
- ID: 239730
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Abstract
We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
About the authors
А. D. Voronova
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Author for correspondence.
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
O. V. Stepanova
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
M. P. Valikhov
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
A. V. Chadin
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
А. S. Dvornikov
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
I. V. Reshetov
University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
Russian Federation, Moscow
V. P. Chekhonin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
Russian Federation, Moscow; Moscow
