电邮这篇文章 Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries
- 作者: Voronova А.1,2, Stepanova O.2, Valikhov M.2, Chadin A.2, Dvornikov А.2, Reshetov I.3, Chekhonin V.1,2
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隶属关系:
- Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
- University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 4 (2018)
- 页面: 523-527
- 栏目: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/239730
- DOI: https://doi.org/10.1007/s10517-018-4025-x
- ID: 239730
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详细
We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.
作者简介
А. Voronova
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
编辑信件的主要联系方式.
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow; Moscow
O. Stepanova
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow
M. Valikhov
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow
A. Chadin
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow
А. Dvornikov
Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow
I. Reshetov
University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow
V. Chekhonin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov National Research Medical University
Email: nastyanastyav@mail.ru
俄罗斯联邦, Moscow; Moscow
