Role of Matrix Metalloproteinase-2 in the Development of Cyclophosphamide-Induced Cardiomyopathy
- Authors: Koldysheva E.V.1, Klinnikova M.G.1, Nikityuk D.B.1, Ivleva E.K.1, Listvyagova N.A.1, Lushnikova E.L.1
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Affiliations:
- Institute of Molecular Pathology and Pathomorphology
- Issue: Vol 164, No 4 (2018)
- Pages: 483-487
- Section: Morphology and Pathomorphology
- URL: https://journals.rcsi.science/0007-4888/article/view/239701
- DOI: https://doi.org/10.1007/s10517-018-4017-x
- ID: 239701
Cite item
Abstract
Immunohistochemical assay was employed to determine localization of MMP-2 in cardiomyocytes of WAG rats and changes in MMP-2 expression during modeled cardiomyopathy induced by single intraperitoneal injection of cyclophosphamide (125 mg/kg) alone or in combination with preventive intraperitoneal administration of an equal dose of asparcam-L (potassium-magnesium asparaginate) 30 min prior to the cytostatic. In the myocardium of control and experimental rats, MMP-2 was mostly located in cardiomyocyte nuclei. During the development of cyclophosphamide-induced cardiomyopathy (in 3 days after injection), the index of MMP-2-positive cardiomyocyte nuclei increased by 76%. In contrast to control hearts, MMP-2 was also expressed in the cardiomyocyte sarcoplasm. Preventive injection of asparcam-L moderated the cardiotoxic effect of cyclophosphamide, which manifested in less pronounced increase in the volume density of cardiomyocytes with lytic changes (by 42%) and index of MMP-2+ cardiomyocyte nuclei (by 23%) in comparison with the rats exposed to cyclophosphamide alone.
About the authors
E. V. Koldysheva
Institute of Molecular Pathology and Pathomorphology
Author for correspondence.
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
M. G. Klinnikova
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
D. B. Nikityuk
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
E. K. Ivleva
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
N. A. Listvyagova
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
E. L. Lushnikova
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk