Email this article Cytokine Production in Mixed Cultures of Mesenchymal Stromal Cells from Wharton’s Jelly and Peripheral Blood Lymphocytes
- Authors: Poltavtsev A.M.1, Poltavtseva R.A.2, Yushina M.N.2, Volgina N.E.1, Svirshchevskaya E.V.3
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Affiliations:
- Institute of Applied Mechanics, Russian Academy of Sciences
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Issue: Vol 163, No 1 (2017)
- Pages: 169-175
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238732
- DOI: https://doi.org/10.1007/s10517-017-3759-1
- ID: 238732
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Abstract
We compared the production of 19 humoral factors in mixed cultures of mesenchymal stromal cells from Wharton’s jelly and allogenic peripheral blood lymphocytes. For evaluation of the specificity of immunosuppressive activity of mesenchymal stromal cells, comparative analysis of the production of these humoral factors in mixed cultures of lymphocytes and epithelial BxPC-3 cells was conducted. The production of soluble factors in both mono- and mixed cultures significantly correlated (p<0.05). The maximum production was found for proinflammatory chemokine IP-10 and IFN-γ and anti-inflammatory cytokine IL-10. The major difference of mesenchymal stromal cells from epithelial BxPC-3 cells was 7-fold higher production of IL-10, which can explain the immunosuppressive effect of mesenchymal stromal cells.
About the authors
A. M. Poltavtsev
Institute of Applied Mechanics, Russian Academy of Sciences
Email: esvir@mx.ibch.ru
Russian Federation, Moscow
R. A. Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: esvir@mx.ibch.ru
Russian Federation, Moscow
M. N. Yushina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: esvir@mx.ibch.ru
Russian Federation, Moscow
N. E. Volgina
Institute of Applied Mechanics, Russian Academy of Sciences
Email: esvir@mx.ibch.ru
Russian Federation, Moscow
E. V. Svirshchevskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: esvir@mx.ibch.ru
Russian Federation, Moscow
