Отправить статью по E-mail Effect of Fibroblast-Like Cells of Mesenchymal Origin of Cytotoxic Activity of Lymphocytes against NK-Sensitive Target Cells
- Авторы: Lupatov A.1,2, Kim Y.1,2, Bystrykh O.2, Vakhrushev I.1,2, Pavlovich S.2, Yarygin K.1,2, Sukhikh G.1,2
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Учреждения:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Выпуск: Том 162, № 4 (2017)
- Страницы: 552-557
- Раздел: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238371
- DOI: https://doi.org/10.1007/s10517-017-3658-5
- ID: 238371
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Аннотация
We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity. This suggests that fibroblast-like cells can suppress not only isolated NK cells, but also NK cells in natural cell microenvironment.
Об авторах
A. Lupatov
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Автор, ответственный за переписку.
Email: alupatov@inbox.ru
Россия, Moscow; Moscow
Ya. Kim
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow; Moscow
O. Bystrykh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow
I. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow; Moscow
S. Pavlovich
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow; Moscow
G. Sukhikh
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Россия, Moscow; Moscow
