Email this article Effect of Fibroblast-Like Cells of Mesenchymal Origin of Cytotoxic Activity of Lymphocytes against NK-Sensitive Target Cells
- Authors: Lupatov A.Y.1,2, Kim Y.S.1,2, Bystrykh O.A.2, Vakhrushev I.V.1,2, Pavlovich S.V.2, Yarygin K.N.1,2, Sukhikh G.T.1,2
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Affiliations:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 162, No 4 (2017)
- Pages: 552-557
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238371
- DOI: https://doi.org/10.1007/s10517-017-3658-5
- ID: 238371
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Abstract
We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity. This suggests that fibroblast-like cells can suppress not only isolated NK cells, but also NK cells in natural cell microenvironment.
About the authors
A. Yu. Lupatov
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: alupatov@inbox.ru
Russian Federation, Moscow; Moscow
Ya. S. Kim
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow; Moscow
O. A. Bystrykh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow
I. V. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow; Moscow
S. V. Pavlovich
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow
K. N. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow; Moscow
G. T. Sukhikh
V. N. Orekhovich Research Institute of Biomedical Chemistry; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: alupatov@inbox.ru
Russian Federation, Moscow; Moscow
