Email this article Expression of Therapeutic Gene FCU1 Sensitizes Pancreatic Cancer Cells to 5-Fluorocytosine and Enhances the Cytotoxic Effect of 5-Fluorouracil
- Authors: Kopantsev E.P.1, Kostina M.B.1, Grankina E.V.2, Kopantseva M.R.1, Egorov V.I.3, Sverdlov E.D.1
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Affiliations:
- Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- A. V. Vishnevsky Institute of Surgery, Ministry of the Health of the Russian Federation
- Municipal Clinical Hospital No. 5, Moscow City Health Department
- Issue: Vol 161, No 6 (2016)
- Pages: 808-810
- Section: Genetics
- URL: https://journals.rcsi.science/0007-4888/article/view/237803
- DOI: https://doi.org/10.1007/s10517-016-3517-9
- ID: 237803
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Abstract
Hybrid therapeutic gene FCU1 gene was cloned into a lentiviral expression vector and the therapeutic effect of its expression was studied in three pancreatic cancer cell lines. Expression of FCU1 gene sensitized cells of two of three studied pancreatic cancer cell lines to 5-fluorocytosine. In addition, uracil phosphoribosyl transferase activity of the hybrid FCU1 protein increased sensitivity of transfected cells of all three studied pancreatic cancer cell lines to 5-fluorouracil, a standard chemotherapeutic agent.
About the authors
E. P. Kopantsev
Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: kopantzev@ibch.ru
Russian Federation, Moscow
M. B. Kostina
Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kopantzev@ibch.ru
Russian Federation, Moscow
E. V. Grankina
A. V. Vishnevsky Institute of Surgery, Ministry of the Health of the Russian Federation
Email: kopantzev@ibch.ru
Russian Federation, Moscow
M. R. Kopantseva
Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kopantzev@ibch.ru
Russian Federation, Moscow
V. I. Egorov
Municipal Clinical Hospital No. 5, Moscow City Health Department
Email: kopantzev@ibch.ru
Russian Federation, Moscow
E. D. Sverdlov
Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kopantzev@ibch.ru
Russian Federation, Moscow
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