Email this article Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium
- Authors: Aleksandrova A.V.1, Burmistrova O.A.1, Fomicheva K.A.2, Sakharov D.A.1,3
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Affiliations:
- BioClinicum Research and Production Center
- National Medical Research Radiology Center
- Moscow State University of Mechanical Engineering (MAMI)
- Issue: Vol 161, No 1 (2016)
- Pages: 120-124
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237180
- DOI: https://doi.org/10.1007/s10517-016-3360-z
- ID: 237180
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Abstract
We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.
About the authors
A. V. Aleksandrova
BioClinicum Research and Production Center
Author for correspondence.
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
O. A. Burmistrova
BioClinicum Research and Production Center
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
K. A. Fomicheva
National Medical Research Radiology Center
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow
D. A. Sakharov
BioClinicum Research and Production Center; Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
Russian Federation, Moscow; Moscow
