Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium
- Авторлар: Aleksandrova A.V.1, Burmistrova O.A.1, Fomicheva K.A.2, Sakharov D.A.1,3
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Мекемелер:
- BioClinicum Research and Production Center
- National Medical Research Radiology Center
- Moscow State University of Mechanical Engineering (MAMI)
- Шығарылым: Том 161, № 1 (2016)
- Беттер: 120-124
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237180
- DOI: https://doi.org/10.1007/s10517-016-3360-z
- ID: 237180
Дәйексөз келтіру
Аннотация
We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.
Авторлар туралы
A. Aleksandrova
BioClinicum Research and Production Center
Хат алмасуға жауапты Автор.
Email: a.aleksandrova@bioclinicum.com
Ресей, Moscow
O. Burmistrova
BioClinicum Research and Production Center
Email: a.aleksandrova@bioclinicum.com
Ресей, Moscow
K. Fomicheva
National Medical Research Radiology Center
Email: a.aleksandrova@bioclinicum.com
Ресей, Moscow
D. Sakharov
BioClinicum Research and Production Center; Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
Ресей, Moscow; Moscow
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