Email this article Effects of Deep Cooling and Re-Warming on Ionotropic Glutamatergic Receptors In Vitro
- Authors: Mokrushin A.A.1
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Affiliations:
- Laboratory of Regulation for Functions of Brain Neurons, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences
- Issue: Vol 161, No 1 (2016)
- Pages: 28-31
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237061
- DOI: https://doi.org/10.1007/s10517-016-3337-y
- ID: 237061
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Abstract
We studied the effects of cooling to -10°C and re-warming to 37°C on slices of rat olfactory cortex. The amplitudes of action potential in the lateral olfactory tract and excitatory postsynaptic potential activated by AMPA recovered during slow cooling/re-warming (0.1°C/min), while during rapid cooling/re-warming (9°C/min), they surpassed the control values. NMDA receptor-dependent mechanism was blocked in both cooling/re-warming modes. Swelling of the brain slices was observed during re-warming, especially during rapid cooling/re-warming. Nerve fibers of the lateral olfactory tract and AMPA-related processes survived deep cooling/re-warming, while NMDA-related processes were irreversibly blocked.
About the authors
A. A. Mokrushin
Laboratory of Regulation for Functions of Brain Neurons, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences
Author for correspondence.
Email: mok@inbox.ru
Russian Federation, St. Petersburg
