Complement-Mediated Death of Ciliate Tetrahymena pyriformis Caused by Human Blood Serum
- Authors: Ivanov P.A.1, Faktor M.I.1, Karpova N.S.1, Cheremnykh E.G.1, Brusov O.S.1
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Affiliations:
- Mental Health Research Center
- Issue: Vol 160, No 6 (2016)
- Pages: 775-778
- Section: Microbiology and Immunology
- URL: https://journals.rcsi.science/0007-4888/article/view/236938
- DOI: https://doi.org/10.1007/s10517-016-3307-4
- ID: 236938
Cite item
Abstract
Toxicity of human blood serum for ciliate Tetrahymena pyriformis is determined by the complement system. When ciliate are dying after being exposed to blood serum, cell membrane permeability for low-molecular-weight compounds significantly increases, probably due to pore formation. Serine protease inhibitors or exposure to physical factors inducing complement inactivation (e.g., heating up to 56°C) completely prevented ciliate death under the effect of human serum. Activation of serum complement upon interaction with Tetrahymena cells occurred by the classical or lectin pathway, while the contribution of the alternative activation pathway was negligible.
About the authors
P. A. Ivanov
Mental Health Research Center
Author for correspondence.
Email: ivanovpa@mail.ru
Russian Federation, Moscow
M. I. Faktor
Mental Health Research Center
Email: ivanovpa@mail.ru
Russian Federation, Moscow
N. S. Karpova
Mental Health Research Center
Email: ivanovpa@mail.ru
Russian Federation, Moscow
E. G. Cheremnykh
Mental Health Research Center
Email: ivanovpa@mail.ru
Russian Federation, Moscow
O. S. Brusov
Mental Health Research Center
Email: ivanovpa@mail.ru
Russian Federation, Moscow