Analysis of Plasma microRNA Associated with Hemolysis
- Authors: Shkurnikov M.Y.1,2, Knyazev E.N.1, Fomicheva K.A.2, Mikhailenko D.S.2, Nyushko K.M.2, Saribekyan E.K.2, Samatov T.R.1, Alekseev B.Y.2
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Affiliations:
- BioClinicum Research and Development Center
- National Medical Research Radiological Center, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 6 (2016)
- Pages: 748-750
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/236927
- DOI: https://doi.org/10.1007/s10517-016-3300-y
- ID: 236927
Cite item
Abstract
We analyzed the effect of hemolysis on microRNA profi le of blood plasma. It was found that hemolysis of ~0.05% erythrocytes in a sample signifi cantly affected the concentration of 9 microRNA: hsa-miR-486-5p, hsa-miR-16-5p, hsa-miR-451a, hsa-miR-106a-5p, hsa-miR-17-5p, hsa-miR-93-5p, hsa-miR-20a-5p, hsa-miR-107, and hsa-miR-20b-5p. The effect of hemolysis on plasma content of miR-17 family microRNA was demonstrated.
About the authors
M. Yu. Shkurnikov
BioClinicum Research and Development Center; National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow; Moscow
E. N. Knyazev
BioClinicum Research and Development Center
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
K. A. Fomicheva
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
D. S. Mikhailenko
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
K. M. Nyushko
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
E. K. Saribekyan
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
T. R. Samatov
BioClinicum Research and Development Center
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow
B. Ya. Alekseev
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow