Analysis of Plasma microRNA Associated with Hemolysis

M. Yu. Shkurnikov; E. N. Knyazev; K. A. Fomicheva; D. S. Mikhailenko; K. M. Nyushko; E. K. Saribekyan; T. R. Samatov; B. Ya. Alekseev

doi:10.1007/s10517-016-3300-y

Analysis of Plasma microRNA Associated with Hemolysis

Authors: Shkurnikov M.Y.¹^,2, Knyazev E.N.¹, Fomicheva K.A.², Mikhailenko D.S.², Nyushko K.M.², Saribekyan E.K.², Samatov T.R.¹, Alekseev B.Y.²
Affiliations:
1. BioClinicum Research and Development Center
2. National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Issue: Vol 160, No 6 (2016)
Pages: 748-750
Section: Biophysics and Biochemistry
URL: https://journals.rcsi.science/0007-4888/article/view/236927
DOI: https://doi.org/10.1007/s10517-016-3300-y
ID: 236927

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Abstract
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Abstract

We analyzed the effect of hemolysis on microRNA profi le of blood plasma. It was found that hemolysis of ~0.05% erythrocytes in a sample signifi cantly affected the concentration of 9 microRNA: hsa-miR-486-5p, hsa-miR-16-5p, hsa-miR-451a, hsa-miR-106a-5p, hsa-miR-17-5p, hsa-miR-93-5p, hsa-miR-20a-5p, hsa-miR-107, and hsa-miR-20b-5p. The effect of hemolysis on plasma content of miR-17 family microRNA was demonstrated.

About the authors

M. Yu. Shkurnikov

BioClinicum Research and Development Center; National Medical Research Radiological Center, Ministry of Health of the Russian Federation

Author for correspondence.
Email: mshkurnikov@imscs.msu.ru
Russian Federation, Moscow; Moscow