Email this article Quantitative PCR studies of Aggregatibacter actinomycetemcomitans and Treponema denticola/Tanerella forsythensis Complex as Etiological Factors of Chronic Periodontitis
- Authors: Yanushevich O.O.1, Ayvazova R.A.1, Shibaeva A.V.2,3, Rebrikov D.V.4, Trubnikova E.V.5, Kudykina Y.K.3, Zylnikova M.V.3,6, Zaripova R.S.6, Shevelev A.B.3
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Affiliations:
- A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation
- N. M. Emanuel Institute of Biochemical Physics, the Russian Academy of Sciences
- M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences
- N. I. Vavilov Institute of Common Genetics, the Russian Academy of Sciences
- Kursk State University
- Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources
- Issue: Vol 160, No 4 (2016)
- Pages: 495-497
- Section: Experimental Methods for Clinical Practice
- URL: https://journals.rcsi.science/0007-4888/article/view/236754
- DOI: https://doi.org/10.1007/s10517-016-3205-9
- ID: 236754
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Abstract
Real-time quantitative PCR (Dentofl or kit) was used to detect DNA of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. A bimodal distribution of patients by periodontium colonization with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis, and T. denticola was demonstrated. A new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of pathological colonization level, led to classification of all cases with chronic generalized periodontitis into 3 groups: associated with A. actinomycetemcomitans, with T. forsythensis/T. denticola complex, and cases of uncertain genesis.
About the authors
O. O. Yanushevich
A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation
Email: a_reg@mail.ru
Russian Federation, Moscow
R. A. Ayvazova
A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation
Author for correspondence.
Email: a_reg@mail.ru
Russian Federation, Moscow
A. V. Shibaeva
N. M. Emanuel Institute of Biochemical Physics, the Russian Academy of Sciences; M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences
Email: a_reg@mail.ru
Russian Federation, Moscow; Moscow
D. V. Rebrikov
N. I. Vavilov Institute of Common Genetics, the Russian Academy of Sciences
Email: a_reg@mail.ru
Russian Federation, Moscow
E. V. Trubnikova
Kursk State University
Email: a_reg@mail.ru
Russian Federation, Kursk
Yu. K. Kudykina
M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences
Email: a_reg@mail.ru
Russian Federation, Moscow
M. V. Zylnikova
M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences; Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources
Email: a_reg@mail.ru
Russian Federation, Moscow; Naberezhnye Chelny
R. S. Zaripova
Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources
Email: a_reg@mail.ru
Russian Federation, Naberezhnye Chelny
A. B. Shevelev
M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences
Email: a_reg@mail.ru
Russian Federation, Moscow
