Nephroprotective Effect Exogenous Antioxidant Enzymes during Ischemia/Reperfusion-Induced Damage of Renal Tissue

Abstract

Nephroprotective effect of exogenous chimeric antioxidant enzyme with combined superoxide dismutase and peroxide activities (PSH protein) was studied on the model of ischemia/reperfusion damage of the renal tissue. It was shown that post-ischemic (25- and 45-min ischemia) intravenous administration of PSH protein significantly normalized the levels of creatinine and urea. Histological studies showed that as distinct from ischemic kidney, the structure of renal corpuscles and tubules remained unchanged, the number of atrophied glomeruli and glomeruli with exudates and protein inclusions decreased in the capsular teeth after postischemic intravenous administration of PSH protein. Immunohistochemical investigations showed that post-ischemic intravenous injection of PSH protein significantly reduced the intensity of apoptosis in ischemic renal tissues.