Automated Reticulocyte Counting in Peripheral Blood Smears


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Аннотация

The results of reticulocyte counting obtained using the Vision Hema® Reticulocytes (Vision Hema® RET) software are presented and compared to the results obtained by manual reticulocyte counting. Blood samples with pronounced reticulocytosis (main group, n = 15) and samples with normal cell count (control group, n = 15) are analyzed. Use of a scanner analyzer allows the reticulocyte counting time in the samples of the main group to be reduced by 90.5% (from 202.3 ± 44.7 to 106.3 ± 18.3 s); in the control group it is reduced by 56% (from 155.1 ± 38.0 to 99.9 ± 16.8 s). The results of reticulocyte counting using the Vision Hema® RET software do not differ from the light microscopy data in the main group (p = 0.211) and in the control group (p = 0.53). The Spearman’s correlation coefficient R is 0.977. The results of evaluation of the within-run precision (CVWR) demonstrate high reproducibility of reticulocyte count as compared to the manual method in cases of both normal (9.45% against 20.5%) and high (4.43% against 8.63%) reticulocyte counts. The Vision Hema® RET soft-ware is recommended for use in clinical diagnostic laboratories.

Авторлар туралы

D. Sosnin

Academician E. A. Wagner Perm State Medical University, Ministry of Health of the Russian Federation

Хат алмасуға жауапты Автор.
Email: sosnin_dm@mail.ru
Ресей, Perm

L. Onjanova

OOO West Medica

Email: sosnin_dm@mail.ru
Ресей, Perm

B. Falkov

OOO West Medica

Email: sosnin_dm@mail.ru
Ресей, Perm

O. Kubarev

Academician E. A. Wagner Perm State Medical University, Ministry of Health of the Russian Federation

Email: sosnin_dm@mail.ru
Ресей, Perm

N. Pozdin

Academician E. A. Wagner Perm State Medical University, Ministry of Health of the Russian Federation

Email: sosnin_dm@mail.ru
Ресей, Perm

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