Kinetics and mechanisms of oxidative hemolysis of erythrocytes in the presence of azoand peroxide initiator

The kinetics of oxidative hemolysis of a 0.2% suspension of mouse erythrocytes in the presence of radicalforming initiators, 2,2'-azobis(2-amidinopropane) dihydrochloride and tert-butyl hydroperoxide, was studied over a wide range of concentrations. Hemolysis of erythrocytes proceeded with the activation of lipid peroxidation in erythrocyte membranes. In the case of tert-butyl hydroperoxide, oxidative processes also developed in the soluble part of the cell, leading to the formation of insoluble hemoglobin aggregates. An induction period of hemolysis (the time period required to reach 10% hemolysis) in the presence of 2,2'-azobis(2-amid-inopropane) dihydrochloride decreases inversely proportional to the square root of the initiator concentration, that is in accord with the classical theory of radical chain oxidation of hydrocarbons. In the case of tertbutyl hydroperoxide, this association was not found. The use of 2,2'-azobis(2-amidinopropane) dihydrochloride as a lipid peroxidation initiator is preferred in the erythrocyte model for testing natural and synthetic compounds for antioxidant activity.

erythrocytes, hemoglobin, oxidative hemolysis, lipid peroxidation