Quantitative determination of natural rubber content of Taraxacum kok-saghyz E. Rodin plants using spin probe method of electron paramagnetic resonance spectroscopy
- Autores: Martirosyan L.1,2, Goldberg V.1, Barashkova I.2, Kasparov V.1, Martirosyan Y.1,2, Motyakin M.1,3, Gaydamaka S.4, Varfolomeev S.1,4
-
Afiliações:
- Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
- All-Russian Research Institute of Agricultural Biotechnology
- Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
- Lomonosov Moscow State University
- Edição: Volume 68, Nº 4 (2023)
- Páginas: 730-735
- Seção: Articles
- URL: https://journals.rcsi.science/0006-3029/article/view/142127
- DOI: https://doi.org/10.31857/S0006302923040130
- EDN: https://elibrary.ru/KLNBLV
- ID: 142127
Citar
Resumo
The paper presents a method to carry out a quantitative analysis of the content of natural rubber of Taraxacum kok-saghyz E. Rodin by measuring adsorption of the nitroxide radical TEMPO in root preparations of the said plant. The method is based on a comparison between the integrated intensity of an EPR signal of interest and a standard. The developed method of analysis makes it possible to quickly and with good accuracy determine the content of rubber of plant tissues without its extraction from rubber-containing biomass.
Sobre autores
L. Martirosyan
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
Moscow, Russia
V. Goldberg
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
I. Barashkova
All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
V. Kasparov
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
Yu. Martirosyan
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
Moscow, Russia
M. Motyakin
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
S. Gaydamaka
Lomonosov Moscow State University
Email: levon-agro@mail.ru
Moscow, Russia
S. Varfolomeev
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;Lomonosov Moscow State University
Autor responsável pela correspondência
Email: levon-agro@mail.ru
Moscow, Russia;Moscow, Russia
Bibliografia
- M. Salehi, et al., Industrial Crops and Products, 170, 113667 (2021).
- А. Ю. Америк и др., С.-х. биология, 57 (1), 3 (2022).
- Б. Р. Кулуев, Р. Р. Гарафутдинов, И. В. Максимов и др., Биомика, 2015, Том 7, № 4, С. 224-283.
- Goodyear to develop domestic source of natural rubber, https://www.prnewswire.com/news-releases/good-year-to-develop-domestic-source-of-natural-rubber-301519783.html
- A. Y. Amerik, et al., Rus. J. Plant Physiol., 68 (1), 31 (2021).
- M. Salehi, et al., Industrial Crops and Products, 178, 114562 (2022).
- А. А. Прокофьев, Анализ каучуконосных растений, под ред. А. А. Ничипоровича (Всесоюз. науч.-иссл. ин-т каучука и гуттаперчи, Глав. ред. хим. лит-ры, Москва, 1936), т. 1.
- L. T. Black, et al., Rubber Chemistry and Technology, 56 (2), 367 (1983).
- M. E. Salvucci, T. A. Coffelt, and K. Cornish, Industrial Crops and Products, 30 (1), 9 (2009).
- C. H. Pearson, K. Cornish, and D. J. Rath, Industrial crops and products, 43, 506 (2013).
- S. Azadi, et al., Physiol. Mol. Biol. Plants, 26 (10) 2047 (2020).
- K. Cornish, M. D. Myers, and S. S. Kelley, Industrial Crops and Products, 19 (3), 283 (2004).
- M. Taurines, et al., Industrial Crops and Products, 134, 177 (2019).
- M. C. Davis and Y. Huang, Methods for quantifying rubber content in a plant with NMR: пат. 10578567 США (2020).
- А. М. Вассерман и А. Л. Коварский, Спиновые метки и зонды в физикохимии полимеров (Наука, М., 1986).
- А. Н. Тихонов, Соросовский образоват. журн., № 1, 8 (1998).
- I. I. Barashkova, et al., Appl. Magn. Resonance, 46 (12), 1421 (2015).
- M. A. Uddin, et al., J. Polymer Sci., 58 (14), 1924 (2020).
- В. В. Птушенко, Природа, № 6, 53 (2011).
- Ю. Ц. Мартиросян и др., Аэропонный фитотрон. Патент № RU 196013 U1 (2020).