Email this article Quantitative determination of natural rubber content of Taraxacum kok-saghyz E. Rodin plants using spin probe method of electron paramagnetic resonance spectroscopy
- Authors: Martirosyan L.Y.1,2, Goldberg V.M1, Barashkova I.I2, Kasparov V.V1, Martirosyan Y.T.1,2, Motyakin M.V1,3, Gaydamaka S.N4, Varfolomeev S.D1,4
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Affiliations:
- Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
- All-Russian Research Institute of Agricultural Biotechnology
- Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
- Lomonosov Moscow State University
- Issue: Vol 68, No 4 (2023)
- Pages: 730-735
- Section: Articles
- URL: https://journals.rcsi.science/0006-3029/article/view/142127
- DOI: https://doi.org/10.31857/S0006302923040130
- EDN: https://elibrary.ru/KLNBLV
- ID: 142127
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Abstract
The paper presents a method to carry out a quantitative analysis of the content of natural rubber of Taraxacum kok-saghyz E. Rodin by measuring adsorption of the nitroxide radical TEMPO in root preparations of the said plant. The method is based on a comparison between the integrated intensity of an EPR signal of interest and a standard. The developed method of analysis makes it possible to quickly and with good accuracy determine the content of rubber of plant tissues without its extraction from rubber-containing biomass.
About the authors
L. Yu Martirosyan
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
Moscow, Russia
V. M Goldberg
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
I. I Barashkova
All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
V. V Kasparov
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
Yu. Ts Martirosyan
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;All-Russian Research Institute of Agricultural Biotechnology
Email: levon-agro@mail.ru
Moscow, Russia
M. V Motyakin
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences
Email: levon-agro@mail.ru
Moscow, Russia
S. N Gaydamaka
Lomonosov Moscow State University
Email: levon-agro@mail.ru
Moscow, Russia
S. D Varfolomeev
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;Lomonosov Moscow State University
Author for correspondence.
Email: levon-agro@mail.ru
Moscow, Russia;Moscow, Russia
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