Comparative analysis of the degree of hydrolysis of biopolymers in hyaline cartilage homogenates in the presence of proteolytic enzymes

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

Investigating the degree of hydrolysis in the presence of different proteolytic enzymes is one of the tasks regarding further development of nutraceuticals obtained from connective tissues for biomedicine. Hydrolysis of biopolymers in cattle and swine tracheal hyaline cartilage homogenates was carried out in the presence of the following enzymes: pancreatin, chymopsin, papain and karipazim, a drug that contains papain, a proteolytic enzyme. This study shows that karipazim manufactured by MedFlorina LLC has better efficacy than karipazim manufactured by ZAO Vifiteh, and the greatest degree of hydrolysis for collagen occurrs at 60°C and karipazim concentration of 10%. More complete hydrolysis of proteoglycans was performed in the cattle hyaline cartilage homogenates, because the analysis of NMR spectra revealed the glucose, the final product of glycosaminoglycan hydrolysis.

About the authors

T. I Nikolaeva

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: tomivnik@yandex.ru
Pushchino, Moscow Region, Russia

D. A Barsuk

Pushchino Branch of the Russian Biotechnologycal University

Pushchino, Moscow Region, Russia

M. V Molchanov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

D. A Prokhorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

V. I. Emelyanenko

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

P. V Shekhovtsov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

References

  1. E. J. Miller, in Collagen (CRC Press, Boca Raton, 1988), Vol. 1, P. 139.
  2. E. J. Kucharz, The collagen: biochemistry and pathophysiogy (Springer-Verlag, Berlin, 1992).
  3. B. Brodsky and J. A. M. Ramshaw, Matrix Biol., 15, 545 (1997).
  4. D. J. Prockop and K. I. Kivirikko, Annu. Rev. Biochem., 403 (1995).
  5. Лазерная инженерия хрящей (Физматлит, М., 2006).
  6. M. E. Nimni and R. D. Harkness, in Collagen, (Boca Raton, CRC Press, 1988), Vol. 1, P. 1.
  7. J. E. Scott, Biochem. J., 252, 313 (1988).
  8. A. Veis and A. George, in Extracellular matrix assembly and structure (Acad. Press, N.-Y., 1994), P. 15.
  9. Б. Альбертс, Д. Брей, Дж. Льюис и др. Молекулярная биология клетки (Мир, М.,1987).
  10. В. А. Тутельян, В. Х Хавинсон и В. В Малинин., Бюл. эксперим. биологии и медицины, 135 (1), 1, (2003).
  11. T. Aignera and J. Stove, Adv. Drug Delivery Rev., 55 (1), 569 (2003).
  12. В. Н. Павлова, Т. Т. Копьева, Л. И. Слуцкий и Г. Г. Павлов, Хрящ (Медицина, М., 1988).
  13. V. S. Simons and G. L. Steinmeyer, J. Sci. Rep., 8, 17733 (2018). doi: 10.1038/s41598-018-36046-3
  14. Н. Song and В. Li, Biomed. Sci. Tech. Res., 1 (2), (2017).
  15. Т. Н. Пивненко, Г. Ю. Клычкова, Н. Н. Ковалев и др., Патент РФ №2250047, БИ № 41 (2005).
  16. M. Ahmed, A. K. Verma, and R. Patel, Sustainable Chemistry and Pharmacy, 100315 (2020). doi: 10.1016/j.scp.2020.100315
  17. Т. И. Николаева, М. В. Молчанов, К. С. Лауринавичюс и др. Междунар. журн. приклад. и фундамент. исследований, 10 (3), 442 (2016).
  18. Т. И. Николаева, К. С. Лауринавичюс, В. В. Капцов и др., Бюл. эксперим. биологии и медицины, 167 (2), 194, (2019).
  19. В. В. Капцов, Г. Н. Русаков, Ю. А. Илларионов и др., Патент РФ № 2035855, БИ № 15 (1995).
  20. Л. Я. Телишевская, Белковые гидролизаты. Получение, состав, применение (Аграрная наука, М., 2000).
  21. Q. Wang, Р. Ma, X. Ma, and D. J. Fan, Chem. Pharm. Res., 6, 278 (2014).
  22. S. Iltchenco, A. P. Kempka, R. C. Prestes, and R. Bras. Tecnol. Agroindustr., 11 (1), 2165 (2017).
  23. А. Н. Михайлов, Коллаген кожного покрова и основы его переработки (Легкая индустрия, М., 1971).

Copyright (c) 2023 Russian Academy of Sciences

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies