Comparative analysis of the degree of hydrolysis of biopolymers in hyaline cartilage homogenates in the presence of proteolytic enzymes

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Resumo

Investigating the degree of hydrolysis in the presence of different proteolytic enzymes is one of the tasks regarding further development of nutraceuticals obtained from connective tissues for biomedicine. Hydrolysis of biopolymers in cattle and swine tracheal hyaline cartilage homogenates was carried out in the presence of the following enzymes: pancreatin, chymopsin, papain and karipazim, a drug that contains papain, a proteolytic enzyme. This study shows that karipazim manufactured by MedFlorina LLC has better efficacy than karipazim manufactured by ZAO Vifiteh, and the greatest degree of hydrolysis for collagen occurrs at 60°C and karipazim concentration of 10%. More complete hydrolysis of proteoglycans was performed in the cattle hyaline cartilage homogenates, because the analysis of NMR spectra revealed the glucose, the final product of glycosaminoglycan hydrolysis.

Sobre autores

T. Nikolaeva

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: tomivnik@yandex.ru
Pushchino, Moscow Region, Russia

D. Barsuk

Pushchino Branch of the Russian Biotechnologycal University

Pushchino, Moscow Region, Russia

M. Molchanov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

D. Prokhorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

V. Emelyanenko

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

P. Shekhovtsov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

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