Spectrophotometric determination of sodium-copper chlorophyllin level in lymphocytes
- Authors: Romodin L.A1,2
-
Affiliations:
- State Scientific Center of the Russian Federation - A.I. Burnazyan Federal Medical Biophysical Center of the Federal Medical Biological Agency of Russia
- Russian Biotechnological University (ROSBIOTECH)
- Issue: Vol 68, No 4 (2023)
- Pages: 660-666
- Section: Articles
- URL: https://journals.rcsi.science/0006-3029/article/view/142119
- DOI: https://doi.org/10.31857/S0006302923040051
- EDN: https://elibrary.ru/KJSRDS
- ID: 142119
Cite item
Abstract
This study raises the question of whether it is possible to determine the appearance of sodium-copper chlorophyllin in lymphocytes. To answer this question, the content of chlorophyllin was determined in lymphocyte lysate previously incubated in a medium containing 300 uM of copper chlorophyllin. Significant amounts of chlorophyllin were observed in the lysate, thus, suggesting that chlorophyllin is able to enter cells. However, the question remains as to whether chlorophyllin can pass through the nuclear membrane. Based on our own data and information obtained from the scientific literature, it can be concluded that the passage of chlorophyllin through the nuclear membrane happens at a much slower rate than through the plasmalemma.
About the authors
L. A Romodin
State Scientific Center of the Russian Federation - A.I. Burnazyan Federal Medical Biophysical Center of the Federal Medical Biological Agency of Russia;Russian Biotechnological University (ROSBIOTECH)
Email: rla2904@mail.ru
Moscow, Russia
References
- T. M. Ong, W. Z. Whong, J. Stewart, and H. E. Brockman, Mutation Res., 173 (2), 111 (1986). doi: 10.1016/0165-7992(86)90086-2
- M. Ozcan, D.Aydemir, M. Bacanli, et al., Biol. Trace Element Res., 199 (12), 4475 (2021). doi: 10.1007/s12011-021-02585-6
- J. P. Kamat, K. K. Boloor, and T. P. Devasagayam, Biochim. Biophys. Acta, 1487 (2-3), 113 (2000). doi: 10.1016/s1388-1981(00)00088-3
- А. В. Поздеев и В. П. Гугало, Вестн. Курской гос. с.-х. академии, № 2, 107 (2012).
- А. В. Поздеев и Н. П. Лысенко, Изв. Междунар. академии аграрного образования, 42 (2), 60 (2018).
- А. В. Поздеев, В. К. Промоненков и Н. П. Лысенко, Ветеринар. медицина, № 1, 42 (2010).
- P. Morales-Ramirez and M. T. Mendiola-Cruz, Mutation Res., 344 (1-2), 73 (1995). doi: 10.1016/0165-1218(95)90041-1
- P. Morales-Ramirez and M. C. Garcia-Rodriguez, Mutation Res., 320 (4), 329 (1994). doi: 10.1016/0165-1218(94)90085-x
- S. S. Kumar, B. Shankar, and K. B. Sainis, Biochim. Biophys. Acta 1672 (2), 100 (2004). doi: 10.1016/j.bbagen.2004.03.002
- S. Zimmering, O. Olvera, M. E. Hernandez, et al., Mutation Res., 245 (1), 47 (1990). doi: 10.1016/0165-7992(90)90024-e
- S. K. Abraham, L. Sarma, and P. C. Kesavan, Mutation Res., 322 (3), 209 (1994). doi: 10.1016/0165-1218(94)90008-6
- Л. А. Ромодин и М. А. Игнатов, Радиац. биология. Радиоэкология 63, (2023). DOI
- A. N.Osipov, N. M.Smetanina, M. V.Pustovalova, et al., Free Radic. Biol. Med., 73, 34 (2014). doi: 10.1016/j.freeradbiomed.2014.04.027
- I. B. Korzeneva, S. V. Kostuyk, L. S. Ershova, et al., Mutation Res., 779, 1 (2015). doi: 10.1016/j.mrfm-mm.2015.05.004
- P. L. Olive, J. P. Banath, and R. E. Durand, Radiation Res., 122 (1), 86 (1990).
- K. D. Walsh and T. A. Kato, Methods Mol. Biol. 2519, 65 (2023). doi: 10.1007/978-1-0716-2433-3_7
- B. B. Gomes, S. B.Barros, E. R. Andrade-Wartha, et al., J. Sci. Food Agriculture, 89, 2003 (2009). doi: 10.1002/jsfa.3681
- L. F. Sciuti, L. H. Z.Cocca, A. R. L. Caires, et al., Chem. Phys. Lett., 706, 652 (2018). doi: 10.1016/j.cplett.2018.07.016
- A. G. Favinha, D. S. Barreiro, J. N. Martins, et al., Spectrochim. Acta. Part A - Mol. Biomol. Spectroscopy, 241, 118644 (2020). doi: 10.1016/j.saa. 2020.118644
- К. Р. Григорян и А. А. Шиладжян, Биоорганич. химия, 43 (3), 257 (2017). DOI: 10.7868/ S0132342317020038
- Г. Б. Постникова и Е. А. Шеховцова, Биохимия, 83 (2), 269 (2018). doi: 10.1134/S0006297918020098
- Y. Yao, B. Zhang, H. Pang, et al., Food Chem., 398, 133875 (2023). doi: 10.1016/j.foodchem.2022.133875
- A. M. A. al. Alamein, H. M. Elwy, and S. H. S. El-Din, Spectrochim. Acta. Part A - Mol. Biomol. Spectroscopy, 206, 37 (2019). doi: 10.1016/j.saa.2018.07.073
- М. Б. Березин и О. И. Койфман, Рос. химич. журн., 61 (4), 42 (2017).
- M. Shayeghi, G. O. Latunde-Dada, J. S. Oakhill, et al., Cell, 122 (5), 789 (2005). doi: 10.1016/j.cell. 2005.06.025
- A. Qiu, M. Jansen, A. Sakaris, et al., Cell, 127 (5), 917 (2006). doi: 10.1016/j.cell.2006.09.041
- N. C. Andrews, Cell Metabolism, 5 (1), (2007). doi: 10.1016/j.cmet.2006.12.004
- M. Sakiyama, H. Matsuo, Y. Toyoda, et al., Human Cell, 34 (4), 1082 (2021). doi: 10.1007/s13577-021-00534-y
- F. Sun, Z. Zhao, M. M. Willoughby, et al., Nature, 610 (7933), 768 (2022). doi: 10.1038/s41586-022-05347-z
- M. Hayes and M. G. Ferruzzi, Nutrition Res., 81, 19 (2020). doi: 10.1016/j.nutres.2020.06.010
- U. Kutay and M. W. Hetzer, Curr. Opin. Cell Biol., 20 (6), 669 (2008). doi: 10.1016/j.ceb.2008.09.010
- V. Archambault, J. Li, V. Emond-Fraser, and M. Larouche, Front. Cell Devel. Biol., 10, 1012768 (2022). doi: 10.3389/fcell.2022.1012768
- S. Y. van der Zanden, M. L. M. Jongsma, A. C. M. Neefjes, et al., Trends Cell Biol., 33, 18 (2022). doi: 10.1016/j.tcb.2022.06.002
- J. Bergonie and L. Tribondeau, Comptes Rendus Hebdomadaires des Seances de l'Academie des Science. 143, 983 (1906).